Journal: EMBO Reports
Article Title: Multifaceted conserved functions of Notch during post-embryonic neurogenesis in the annelid Platynereis
doi: 10.1038/s44319-026-00731-6
Figure Lengend Snippet: ( A – H ) Whole-mount in situ hybridizations for markers of neural progenitors ( A – C ), differentiating neurons ( D , E ), differentiated neurons ( F ) and neuronal subtypes ( G , H ) for LY-411575 treated worms and controls at 2 and 5 dpa, ventral views. Green brackets = ventral nerve cord (VNC); light blue brackets = neuroectoderm; purple arrowheads = neurons of the pygidium and cirri; green arrowheads = ectopic VNC neurons; white asterisks = non-specific staining from glands. Solid black lines delineate the outlines of the samples, black dashed lines correspond to the amputation planes. ( I , J ) Hybridization chain reaction (HCR) for Cdx (purple, 1 and 4) and Elav (cyan, 2 and 4) coupled with immunolabelling for acetylated tubulin (green, 3 and 4) for LY-411575 treated worms and controls at 5 dpa. Ventral views are on top and corresponding virtual transverse sections along the yellow dotted lines (‘ and ‘’, respectively) are at the bottom. Solid white lines delineate the outlines of the samples, white dashed lines correspond to the amputation planes. White brackets = VNC; white arrowheads = circular pygidial nerve; white double arrowheads = cirri nerves. dpa = day(s) post-amputation. Scale bars = 50 μm. Anteroposterior (A/P) and dorsoventral (D/V) axes are represented. All images come from representative samples of two biological replicates. Source data are available online for this figure.
Article Snippet: HCR TM Amplifier: B1-647 , Molecular Instruments, Inc. , –.
Techniques: In Situ, Staining, Hybridization
